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W0138
Sigma
Western Blocker™ Solution
for HRP detection systems
Description
| Application | Western Blocker Solution has been optimized for detection systems using horseradish peroxidase (HRP) conjugates; it is also compatible with alkaline phosphatase based systems. |
| Features and Benefits | • Does not inhibit weak HRP signals, as many milk-based blockers do. • Can be used both as a blocking component and as a probing component. • Supplied ready-to-use; no preparative step is required. |
| General description | Western Blocker Solution is used to block both PVDF and nitrocellulose membranes after proteins have been transferred onto them through dot blotting or Western blotting; blocking is performed to reduce or eliminate nonspecific binding of primary or secondary antibodies. |
| Legal Information | Western Blocker is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. |
Properties
| shelf life | ≥2 yr |
| storage temp. | 2-8°C |
