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Properties
| Related Categories | Blocking Buffers, Buffers and Reagents, Buffers, Reagents, & Stains, Core Bioreagents, General Reagents, |
| shelf life | ≥2 yr |
| storage temp. | 2-8°C |
Description
Application
Western Blocker Solution has been optimized for detection systems using horseradish peroxidase (HRP) conjugates; it is also compatible with alkaline phosphatase based systems.
Features and Benefits
• Does not inhibit weak HRP signals, as many milk-based blockers do.
• Can be used both as a blocking component and as a probing component.
• Supplied ready-to-use; no preparative step is required.
General description
Western Blocker Solution is used to block both PVDF and nitrocellulose membranes after proteins have been transferred onto them through dot blotting or Western blotting; blocking is performed to reduce or eliminate nonspecific binding of primary or secondary antibodies.
Legal Information
Western Blocker is a trademark of Sigma-Aldrich Co. LLC
Price and Availability
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Safety Information
| Symbol |     GHS05, GHS06, GHS08, GHS09 |
| Signal word | Danger |
| Hazard statements | H314-H317-H331-H334-H335-H400-H412 |
| Precautionary statements | P261-P273-P280-P305 + P351 + P338-P310 |
| WGK Germany | 3 |
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