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| Related Categories | Blocking Buffers, Buffers and Reagents, Buffers, Reagents, & Stains, Core Bioreagents, General Reagents, |
| shelf life | ≥2 yr |
| storage temp. | 2-8°C |
Western Blocker Solution has been optimized for detection systems using horseradish peroxidase (HRP) conjugates; it is also compatible with alkaline phosphatase based systems.
• Does not inhibit weak HRP signals, as many milk-based blockers do.
• Can be used both as a blocking component and as a probing component.
• Supplied ready-to-use; no preparative step is required.
Western Blocker Solution is used to block both PVDF and nitrocellulose membranes after proteins have been transferred onto them through dot blotting or Western blotting; blocking is performed to reduce or eliminate nonspecific binding of primary or secondary antibodies.
Western Blocker is a trademark of Sigma-Aldrich Co. LLC
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| Symbol | ![]() ![]() ![]() GHS05, GHS06, GHS08, GHS09 |
| Signal word | Danger |
| Hazard statements | H314-H317-H331-H334-H335-H400-H412 |
| Precautionary statements | P261-P273-P280-P305 + P351 + P338-P310 |
| WGK Germany | 3 |
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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